Extract
[...] Additional question If you present a gene expression analysis: do the authors also show, in a figure or another, the analysis of the corresponding protein quantity and if yes, in which figure or part of figure? Additional question CA-2) If you present an analysis of the quantity of a protein: do the authors also show, in a figure or another, the analysis of the corresponding gene expression and if yes, in which figure or part of figure? The study presents the expression of genes (mRNA) and protein levels of JARID2 in Figure 1. [...]
[...] evaluate the impact of JARID2 suppression on OSCC cell proliferation. The authors sought to determine whether gene suppression reduces cell growth by using siRNA to inhibit JARID2. Question(B-2) What other methodology could have been alternatively chosen to achieve this specific objective? The colony formation assay, which assesses long-term cell growth after gene inactivation or overexpression. The role of JARID2 in proliferation can be measured, in addition to the CCK-8 assay, by comparing the size and number of colonies between treated cells and control cells. [...]
[...] Question complémentaire(CB-2) If you present an analysis of apoptosis: do the authors also show, in a figure or another, the analysis of proliferation of the same cells and if yes, in which figure or part of figure? The authors analyzed the proliferation of OSCC cells in figure 5 by CCK-8 and colony formation assays after JARID2 inactivation, which allows to see the role of JARID2 in the regulation of cell proliferation and apoptosis in oral epidermoid carcinoma cells. References Wang, Y., Wei, Y., Huang, J., Li, X., You, D., Wang, L., & Ma, X. (2024). [...]
[...] Prognostic value of matrix metalloproteinase-2 protein and matrix metalloproteinase-9 protein in colorectal cancer: a meta-analysis - Wang, Y., Wei, Y., Huang, J., Li, X., You, D., Wang, L., & Ma, X. (2024) - Current in vitro methodologies in biomedical research in recent scientific studies Figure or part of a figure analyzing gene expression or the quantity of a protein Figure number or part of a figure in the chosen article Figure panels and F Copy of this figure or part of a figure Complete legend of the figure (and if only a part of a figure is analyzed, highlight in the complete legend of the figure) all the passages relating to the chosen part of the figure, but only these passages) Fig.1 (A)TCGA data show higher expression of JARID2 in OSCC. [...]
[...] Figure or part of figure analyzing cell proliferation or apoptosis Number of this figure or part of figure in the chosen article Figure Panel G Copy of this figure or part of figure Complete legend of the figure (and if it is only part of a figure that is analyzed, underline in the complete legend of the figure all the passages relating to the chosen part of the figure, but only these passages) Fig.1 TCGA data show higher expression of JARID2 in OSCC. Immunohistochemistry: JARID2 is expressed in adjacent normal buccal mucosa and OSCC. Global survival according to JARID2 expression. Higher expression in adjacent normal tissues. RT-qPCR confirms lower expression in normal tissues. JARID2 expression in HACAT, SCC25, HN30. Knockdown and overexpression efficacy validated by RT-qPCR. [...]
