This document provides an analysis of the PYC1 and PYC2 genes in the yeast genome, discussing their role in glucose metabolism and the effects of disrupting these genes on yeast growth. Written for a molecular biology course, this analysis explores the enzymatic reaction catalyzed by PYC proteins and the importance of lysine residue K19 for PYC1 protein activity.
Extract
[...] These sequences do not seem to correspond to the same genes since the ATG codons found in the middle of these sequences are not positioned in the same place. These sequences are therefore not identical. Enzymatic reaction catalyzed by PYC proteins: This reaction can be found on the Uniprot website, on the PYC protein page (Uniprot is a protein database that brings together numerous information on each protein). Question The PYC1 and PYC2 genes are not essential for yeast survival since when one of the two genes is modified, no obvious phenotype is observed in yeast. [...]
[...] Question The most important residue for the activity of the PYC1 protein is lysine at position 19 of the amino acid sequence. This residue corresponds to the codon AAG at position 55-57 of the coding sequence. This residue is important for the activity of the PYC1 protein because it undergoes post-translational modification. Indeed, this residue undergoes succinylation: that is, the addition of a succinyl group that modifies the charge of lysine to cause a change in the structure and function of the protein. [...]
