Carotenoids, long orange carrot, old white carrot, PSY1, PSY2 genes, domestication, nutritional benefits, reducing sugars, lignin content
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Abstract
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Abstract
This document compares the characteristics of long orange carrots and old white carrots, highlighting the presence of carotenoids and their nutritional benefits.
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[...] This means that the old white carrots have a high lignin content unlike the long orange carrots. This explains that the texture of the long orange carrot is preferable to the old white carrot, less fibrous, and with a higher nutritional interest. Finally, according to the experiment to highlight the presence of reducing sugars -such as glucose or saccharose-, carried out according to the protocol in document we can observe a red coloration for both the old white carrot and the long orange carrot. [...]
[...] These results confirm our previous results of pigment separation by chromatography, in which we observed carotenes only in the long orange carrots. - The second and third graphs of document 6 show that the transcription levels of the PSY1 and PSY2 genes are very low in the wild white carrot, medium to low in the cultivated white carrot, and much higher in the orange carrots. We observe in these carrots that the more important their carotenoid content is, the higher the transcription level of their PSY1 and PSY2 genes is. [...]
[...] Thus To conclude, we can see that the domestication of carrots has made it possible to obtain carrots commonly known and referred to as long orange carrots, with characteristics more favorable to humans. These long orange carrots, for which the transcription of the PSY1 and PSY2 genes is much higher than in the old white carrot, thus favor the carotene content of the carrots. Moreover, the domestication of carrots has made it possible to obtain carrots for which we have a clear reduction in lignin content, and an increased content of reducing sugars, thus making the carrot more interesting from a gustatory and nutritional point of view. [...]
[...] This means that the long orange carrot contains carotenoids, unlike the old white carrot. On the other hand, we did not observe the presence of xanthophylls on our chromatography for any of the carrots, so either they were not present in the carrots or the amount of sample deposited was too small to highlight them. In addition to the aesthetic contribution they bring to the carrot, due to the orange coloration, the carotenoids present in the long orange carrot bring an additional nutritional interest for Man. [...]
[...] The results of these experiments are presented in the table below: Tested vegetable Old White Carrot Carrot long orange Results of pigment separation. Migration by chromatography after depositing crushed carrot samples on paper strips, soaked in a solvent, followed by a 20-minute incubation in the dark (protocol according to document Results of lignin stainingine. Revelation of lignin presence by phloroglucinol staining in acidic medium on fine transverse sections of carrots (protocol according to document Results of the highlighting of reducing sugars. [...]
